团队队伍

COLLEGE OF LIFE SCIENCES
团队概况
专家人才
植物生物学
微生物学
生化与分子
动物生物学
实验中心
兼职教授
退休教授
师德师风
高级职称

曾严/教授

发布时间:2022年10月11日 10:04 | 编辑:成丹 | 作者:

本科毕业于中国科技大学,博士毕业于华盛顿大学 (圣 路易斯)。

以人细胞和小鼠为模型研究人及哺乳动物microRNA的生成机制,及microRNA对人疾病的调控机理。在Nature、Molecular Cell、PNAS、EMBO、Nucl. Acids Res.等期刊发表多篇文章。

MEMBERSHIP

American Association for the Advancement of Science

RNA Society

Society for Neuroscience

American Society for Biochemistry and Molecular Biology


教学情况


Fundamental pharmacology (本科生)

生科导论(本科生)

实验动物学大实验(本科生)

RNA research methodologies (硕士、博士研究生)

动物学研究专题(硕士、博士研究生)

生物学研究前沿(博士研究生)



科研情况


Zeng, Y., Weiss, C., Yao, T. T., Huang, J., Siconolfi-Baez, L., Hsu, P., and Rushbrook, J. I. (1995) Isocitrate dehydrogenase from bovine heart: primary structure of subunit 3/4.  Biochem. J. 310: 507-516.

Zeng, Y., Forbes, K. C., Wu, Z., Moreno, S., Piwnica-Worms, H., and Enoch, T. (1998) Replication checkpoint requires phosphorylation of the phosphatase Cdc25 by Cds1 or Chk1.  Nature 395: 507-510.

Zeng, Y. and Piwnica-Worms, H. (1999) DNA damage and replication checkpoints in fission yeast require nuclear exclusion of Cdc25 via 14-3-3 binding.  Mol. Cell. Biol. 19: 7410-7419.

Weiss, C., Zeng, Y., Huang, J., Sobocka, M. B., and Rushbrook, J. I. (2000) Bovine NAD+ dependent isocitrate dehydrogenase: alternative splicing and tissue dependent expression of subunit 1.  Biochemistry 39: 1807-1816.

Zeng, Y., Russell, R.A., Erlwein, O., Cullen, B.R., and McClure, M.O. (2001) The R region found in the Human Foamy Virus long terminal repeat is critical for both Gag and Pol protein expression.  J. Virology 75: 6817-6824.

Wiegand, H. L., Coburn, G. A., Zeng, Y., Kang, Y., Bogerd, H. P., and Cullen, B. R. (2001) Formation of Tap/NXT1 heterodimers activates Tap-dependent nuclear mRNA export by enhancing recruitment to nuclear pore complexes. Mol. Cell. Biol. 22: 245-256.

Zeng, Y., Wagner, E. J., and Cullen, B. R. (2002) Both natural and designed microRNAs can inhibit the expression of cognate mRNAs when expressed in human cells.  Mol. Cell 9: 1327-1333.

Zeng, Y., and Cullen, B. R. (2002) RNA interference in human cells is restricted to the cytoplasm. RNA 8: 855-860.

Zeng, Y., and Cullen, B. R. (2003) Sequence requirements for microRNA processing and function in human cells.  RNA 9: 112-123.

Zeng, Y., Yi, R., and Cullen, B. R. (2003) microRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms.  Proc. Natl. Acad. Sci. U.S.A.  100: 9779-9784.

Zeng, Y., and Cullen, B. R. (2004) Structural requirements for pre-microRNA binding and nuclear export by Exportin 5.  Nucleic Acids Research 32: 4776-4785.

Zeng, Y., Cai, X., and Cullen, B. R. (2005) Using RNA polymerase II to transcribe artificial microRNAs.  Methods in Enzymology 392: 371-380.

Zeng, Y., Yi, R., and Cullen, B. R. (2005) Recognition and cleavage of primary microRNA precursors by the nuclear processing enzyme Drosha.  EMBO J. 24: 138-148.

Zeng, Y., and Cullen, B. R. (2005) Efficient processing of primary microRNA hairpins by Drosha requires flanking nonstructured RNA sequences.  J. Biol. Chem. 280: 27595-27603.

Zeng, Y., and Cullen, B. R. (2006) Recognition and cleavage of primary microRNA transcripts.  Methods Mol. Biol. 342: 49-56.

Zeng, Y., and Cullen, B.R. (2006) The biogenesis and function of microRNAs.  In Gene Expression and Regulation, J. Ma ed. (Higher Education Press/Springer), pp. 481-492.

Zeng, Y. (2006) Principles of microRNA production and maturation.  Oncogene 25: 6156-6162.

Zeng, Y., Sankala, H., Zhang, X., and Graves, P (2008) Phosphorylation of Argonaute2 at serine 387 facilitates its localization to processing bodies. Biochem. J. 413: 429-436.

Kalscheuer, S., Zhang, X., Upadhyaya, P., and Zeng, Y. (2008) Differential expression of microRNAs in early stage neoplastic transformation in the lungs of F344 rats chronically treated with the tobacco carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone.  Carcinogenesis 29: 2394–2399.

Kren, B. T., Wong, P. Y.-P., Sarver, A., Zhang, X., Zeng, Y., and Steer, C. J. (2009) microRNAs identified in highly purified liver-derived mitochondria may play a role in apoptosis.  RNA Biol. 6: 65-72.

Zhang, X., Xu, W, Tan, J., and Zeng, Y. (2009) Stripping custom microRNA microarrays and the lessons learned about probe:slide interactions.  Anal. Biochem. 386: 222-227.

Zhang, X., Graves, P. R., and Zeng, Y. (2009) Stable Argonaute2 overexpression differentially regulates microRNA production.  Biochimica Biophysica Acta 1789: 153-159.

Zeng, Y. (2009) Regulation of the mammalian nervous system by microRNAs.  Mol. Phar. 75: 259-264.

Zeng, Y. (2009) RNAi in mammalian cells.  In RNA Interference: Methods for Plants and Animals, T. Doran and C. Helliwell eds. (CABI International), pp 205-227.

Cunningham, J. M., Oberg, A. L., Borralho, P. M., Kren, B. T., French, A. J., Wang, L., Bot, B. M., Morlan, B. W., Silverstein, K. A. T., Staggs, R., Zeng, Y., Lamblin, A.-F., Hilker, C. A., Fan, J.-B., Steer, C. J., and Thibodeau, S N. (2009) Evaluation of a New High-Dimensional miRNA Profiling Platform.  BMC Medical Genomics 2: 57.

Kren, B. T., Wong, P. Y.-P., Shiota, A., Zhang, X., Zeng, Y., and Steer, C. J. (2009) Polysome trafficking of transcripts and microRNAs in regenerating liver after partial hepatectomy.  Am J Physiol Gastrointest Liver Physiol 297: 1181-1192.

Zheng, H., Zeng, Y., Zhang, X., Chu, J., Loh, H. H., and Law, P.-Y. (2010) µ-Opioid Receptor Agonists Differentially Regulate the Expression of miR-190 and NeuroD.  Mol. Phar. 77: 102-109.

Melkamu, T., Zhang, X., Tan, J., Zeng, Y., and Kassie, F. (2010) Alteration of microRNA expression in vinyl-carbamate-induced mouse lung tumors and modulation by the chemopreventive agent indole-3-carbinol.  Carcinogenesis 31: 252-258.

Zhang, X., and Zeng, Y. (2010) Regulation of mammalian microRNA expression.  J. Cardiovascular Translational Res. 3: 197-203.

Park, C. W., Zeng, Y., and Steer, C. J. (2010) Human DNA methyltransferase 3a does not associate with microRNAs in the regulation of DNA methylation.  J. Cardiovascular Translational Res. 3: 290-295.

Zheng, H., Zeng, Y., Chu, J., Kam, A. Y. F., Loh, H., and Law, P.-Y. (2010) Modulations of NeuroD Activity Contribute to the Differential Effects of Morphine and Fentanyl on Dendritic Spine Stability.  J. Neurosci. 30: 8102-8110.

Chen, Y., Zhou, H., Sarver, A., Zeng, Y., Roy-Chowdhury, J., Steer, C., Sahin, M. B. (2010) Hepatic differentiation of liver derived progenitor cells and microRNA analysis of their in vitro differentiation.  Liver Transplantation 16: 1086-1097.

Zeng, H., Chu, J., Zeng, Y., Loh, H. H., and Law, P.-Y. (2010) Yin Yang 1 Phosphorylation Contributes to the Differential Effects of mu-Opioid Receptor Agonists on MicroRNA-190 Expression .  J. Biol. Chem 285: 21994-22002.

Zhang, X., and Zeng, Y. (2010)  The terminal loop region controls microRNA processing by Drosha and Dicer.  Nucl. Acids Res. 38: 7689-7697.

Castro, R. E., Ferreira, D. M. S., Zhang, X., Borralho, P. M., Sarver, A. L., Zeng, Y., Steer, C. J., Kren, B. T., and Rodrigues, C. M. P. (2010) Identification of microRNAs during rat liver regeneration after partial hepatectomy and modulation by ursodeoxycholic acid.  American Journal of Physiology - Gastrointestinal and Liver Physiology 299: G887-897.

Park, C. W., Zeng, Y., Zhang, X., Subramanian, S., and Steer, C. J. (2010) Mature microRNAs identified in highly purified nuclei from HCT116 colon cancer cells.  RNA Biology 7:606-614. PMID: 20864815.

Ghosh, G., Subramanian, I. V., Adhikari, N., Zhang, X., Joshi, H. P., Basi, D. L., Chandrashekhar, Y. S., Hall, J. L., Roy, S., Zeng, Y., and Ramakrishnan, S. (2010) Hypoxia-induced microRNA-424 expression in endothelial cells regulates HIF-1a isoforms by targeting CUL2 and promotes angiogenesis.  J. Clin. Invest. 120: 4141–4154.

Lu, C., Huang, X., Zhang, X., Roensch, K., Cao, Q., Nakayama, K., Blazar, B. R., Zeng, Y., and Zhou, Z. (2011) miR-221 and miR-155 regulate human dendritic cell development, apoptosis and IL-12 production through targeting of p27kip1, KPC1 and SOCS-1.  Blood 117: 4293-4303.

Shu, J., Kren, B. T., Xia, Z., Wong, P. Y., Li, L., Hanse, E. A., Min, M. X., Li, B., Albrecht, J. H., Zeng, Y., Subramanian, S., and Steer, C. J. (2011) Genome-wide microRNA downregulation as a negative feedback mechanism in the early phases of liver regeneration.  Hepatology 54: 609-619.

Feng, Y., Zhang X., Song, Q., Li, T., and Zeng, Y. (2011) Drosha processing controls the specificity and efficiency of global microRNA expression.  Biochimica Biophysica Acta, 1809: 700-707.

Zhang, X., and Zeng, Y. (2011) Performing custom microRNA microarray experiments.  Journal of Visualized Experiments 56: e3250.

Zeng, Y. (2011) The functional consequences and implications of relative substrate specificity in complex biochemical systems.  Front. Genetics 2: 65.

Feng, Y., Zhang, X., Graves, P., and Zeng, Y. (2012) A comprehensive analysis of precursor microRNA cleavage by human Dicer.  RNA 18: 2083-2092.

Graves, P., and Zeng, Y. (2012) Biogenesis of mammalian microRNAs: a global view.  Genomics Proteomics & Bioinformatics 10: 239-245.

Zhang, X., Graves, P., and Zeng, Y. (2013) Overexpression of human Argonaute2 inhibits cell and tumor growth.  Biochimica Biophysica Acta-General Subjects 1830: 2553–2561.

Banerjee, S., Meng, J., Das, S., Krishnan, A., Haworth, J., Charboneau, R., Zeng, Y., Ramakrishnan, S., and Roy, S. (2013) Morphine induced exacerbation of sepsis is mediated by tempering endotoxin tolerance through modulation of miR-146a. Scientific Reports 3: 1977.

Zheng, Y., Tan, D., Chen, Z., Hu, C., Mao, Z. J., Singleton, T. P., Zeng, Y., Shao, X., and Yin, B. (2014) A Non-genetic approach to labelling acute myeloid leukemia and bone marrow cells with quantum dots. J. Nanosci. Nanotechnol. 14: 4014-4021.

Zeng, Y. (2014) Relative Specificity: all substrates are not created equal.  Genomics Proteomics & Bioinformatics 12: 1-7.

Joshi, H. P., Subramanian, I. V., Ghosh, G., Rupaimoole, R., ,Evans, C., Saluja, M., Jing, Y., Cristina, I., Zeng, Y., Shah, V. H., Sood, A. K., and Ramakrishnan, S. (2014)  Dynamin 2 along with its intron-associated miR-199a reciprocally regulate HIF-1 and metastasis.  Proc. Natl. Acad. Sci. U.S.A. 111 : 5331–5336.

Hu, C., Chen, Z, Zhao, W., Wei, L, Zheng, Y., He, C., Zeng, Y., and Yin, B. (2014) Vesicular Stomatitis Virus G Glycoprotein and ATRA Enhanced Bystander Killing of Chemoresistant Leukemic Cells by Herpes Simplex Virus Thymidine Kinase/Ganciclovir. Biomol Ther. 22: 114-121.

Li, L., Feng, H., Da, Q., Jiang, H., Chen, L., Xie, L., Huang, Q., Xiong, H., Luo, F., Kang, L., Zeng, Y., Hu, H., Hou, W., and Feng, Y. (2016) Expression of HIV-encoded microRNA-TAR and its inhibitory effect on viral replication in human primary macrophages.  Arch Virol. 161(5): 1115-1123

Lin, J., Xia, J. Chen, Y.T., Zhang, K.Y., Zeng, Y., and Yang, Q. (2017) H9N2 avian influenza virus enhances the immune responses of BMDCs by down-regulating miR29c.  Vaccine 35: 729-737.

Zhang, X., Li, P., Lin, J., Huang, H., Yin, B., and Zeng, Y. (2017) The insertion in the double-stranded RNA binding domain of human Drosha is important for its function. Biochim Biophys Acta. 1860: 1179-1188.

Lin,  J., Xia, J., Tu, C. Z., Zhang, K. Y., Zeng, Y., and Yang, Q. (2017) H9N2 avian influenza virus protein PB1 enhances the immune responses of bone marrow-derived dendritic cells by down-regulating miR375. Front Microbiol.  8: 287.

Zhang, X., Hu, S., Su, J., Xie, Z., Li W., and Zeng, Y. (2018) Correlation analyses reveal a limited role of transcription in genome-wide differential microRNA expression in mammals. Front. Genet. 9: 154.

Huang, Q., Chen, L., Luo, M., Lv, H., Luo, D., Li, T., Huang, S., Xie, L., Teng, Y., Liu, Z., Luo, F., Xiong, H., Zeng, Y., Hou, W., and Feng, Y. (2018) HIV-1-Induced miR-146a Attenuates Monocyte Migration by Targeting CCL5 in Human Primary Macrophages.  AIDS Res Hum Retroviruses 34: 580-589.

Teng, Y., Luo, M., Yu, T., Chen, L., Huang, Q., Chen, S., Xie, L., Zeng, Y., Luo, F., Xiong, H., Liu, Y., Hou, W., and Feng, Y. (2019) CRISPR/Cas9-mediated deletion of miR-146a enhances antiviral response in HIV-1 infected cells. Genes Immun.  20: 327-337.

Li, P., Tian, Q., Hu, M., Li, W., Zhang, X., and Zeng, Y. (2019) Cloning, expression, and characterization of the zebrafish Dicer and Drosha enzymes.  Biochem Biophys Res Commun. 514: 200-204.

Li, P., Chen, Y., Juma, C. A., Yang, C., Huang, J., Zhang, X., and Zeng, Y. (2019) Differential inhibition of target gene expression by human microRNAs. Cells 8: 791.


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